ball Antibody

Datasheet

Code	CSB-PA762561XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) ball Polyclonal antibody

Uniprot No.

Q7KRY6

Target Names

ball

Alternative Names

ball antibody; ballchen antibody; nhk-1 antibody; CG6386 antibody; Nucleosomal histone kinase 1 antibody; EC 2.7.11.1 antibody; Protein baellchen antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) ball protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Serine/threonine-protein kinase involved in somatic mitosis and female meiosis. Required for spindle organization in mitosis, and for the establishment or maintenance of meiosis-specific chromosomal configurations, including the prophase I karyosome and the metaphase I spindle. Specifically phosphorylates nucleosomal H2A on 'Thr-119'. Required for the development and organization of indirect flight muscle sarcomeres by regulating the formation of M line and H zone and the correct assembly of thick and thin filaments in the sarcomere.

Gene References into Functions

NHK-1 in chromosome condensation phosphorylates Barrier-to-autointegration factor and suppress its activity in linking chromosomes to nuclear envelope proteins and is important for chromosome condensation during mitosis as well as in oocytes. PMID: 25501812
Data suggest that NHK-1 is a crucial regulator of meiosis and that the meiotic checkpoint suppresses NHK-1 activity to prevent oocyte nuclear reorganisation until DNA breaks are repaired. PMID: 21060809
NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster PMID: 16301329

Subcellular Location

Cytoplasm. Nucleus. Chromosome. Cytoplasm, myofibril, sarcomere, Z line. Cytoplasm, myofibril, sarcomere, M line.

Protein Families

Protein kinase superfamily, CK1 Ser/Thr protein kinase family, VRK subfamily

Tissue Specificity

Expressed in ovaries (at protein level). Expressed in indirect flight muscle (IFM) (at protein level).

Database Links

KEGG: dme:Dmel_CG6386

STRING: 7227.FBpp0084465

UniGene: Dm.1346

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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ball Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background