Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NHX1 Polyclonal antibody

Uniprot No.

Q68KI4

Target Names

NHX1

Alternative Names

NHX1 antibody; At5g27150 antibody; T21B4.60 antibody; Sodium/hydrogen exchanger 1 antibody; Na(+)/H(+) exchanger 1 antibody; NHE-1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana NHX1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Acts in low affinity electroneutral exchange of protons for cations such as Na(+) or K(+) across membranes. Can also exchange Li(+) and Cs(+) with a lower affinity. Involved in vacuolar ion compartmentalization necessary for cell volume regulation and cytoplasmic Na(+) detoxification. Required during leaves expansion, probably to stimulate epidermal cell expansion. Confers competence to grow in high salinity conditions.

Gene References into Functions

Overexpression of AtNHX1 gene not only improved salt tolerance but also drought tolerance in transgenic groundnut. PMID: 21455815
Studies shoe that the rutin content of the roots, stems and leaves of AtNHX1 transgenic buckwheat increased than those of the control plants. PMID: 17366888
Use of the T-DNA insertional mutant of AtNHX1 (nhx1 plants) and DNA arrays to assess differences in transcriptional profiles and further characterize the roles of this protein. PMID: 15546358
the presence of a vacuolar calmodulin-like protein acting on the vacuolar-localized AtNHX1 C terminus in a Ca(2+)- pH-dependent manner suggests the presence of signaling entities acting within the vacuole. PMID: 16249341
AtNHX1 has a role in calcium signaling, sulfur metabolism, cell structure and cell growth, as well as vesicular trafficking and protein processing. PMID: 17411438
To generate salt-tolerant turf and forage, tall fescue (Festuca arundinacea) was transformed with AtNHX1. PMID: 17561307
Poisoning by toxic cations in the endosome/prevacuolar compartment is detrimental for cell functions, but it can be alleviated by improving the discrimination of transported alkali cations by the resident cation/H(+) antiporter. PMID: 19307188

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Subcellular Location

Vacuole membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Tonoplast. Possibly also present in endoplasmic reticulum (ER) and Golgi apparatus.

Protein Families

Monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family

Tissue Specificity

Ubiquitous, with higher levels around vascular tissues and guard cells.

Database Links

KEGG: ath:AT5G27150

STRING: 3702.AT5G27150.1

UniGene: At.25435

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NHX1 Antibody

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