NHX5 Antibody

Code CSB-PA823327XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NHX5 Polyclonal antibody
Uniprot No.
Target Names
NHX5
Alternative Names
NHX5 antibody; At1g54370 antibody; F20D21.19Sodium/hydrogen exchanger 5 antibody; Na(+)/H(+) exchanger 5 antibody; NHE-5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NHX5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in trafficking to the vacuole. Required for cell proliferation and cell expansion, but not for cell differentiation. Acts in low affinity electroneutral exchange of protons for cations such as Na(+) or K(+) across membranes. May also exchange Li(+) and Cs(+) with a lower affinity.
Gene References into Functions
  1. AtNHX5 and AtNHX6 regulate the trafficking of seed storage proteins into the vacuole; the H(+) leak pathway conducted by AtNHX5 and AtNHX6 is critical for protein transport. PMID: 27175802
  2. Study shows that NHX5 and NHX6 are necessary for processing of the predominant seed storage proteins, and also influence the processing and activity of a vacuolar processing enzyme. PMID: 26416852
  3. required for protein storage vacuole localization of the SNARE complex and its function in protein transport PMID: 26986836
  4. NHX5 and NHX6 are putative endosomal Na+ (K+)/H+ antiporters expressed throughout plant development. They are are functionally redundant and play key roles in cell proliferation and cell growth. PMID: 21278129
Subcellular Location
Endosome membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein.
Protein Families
Monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family
Tissue Specificity
Expressed in roots, leaves, stems, flowers and siliques. Detected at low levels in roots and shoots.
Database Links

KEGG: ath:AT1G54370

STRING: 3702.AT1G54370.1

UniGene: At.27336

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