NIP2-1 Antibody

Code CSB-PA744376XA01OFG
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Product Details

Full Product Name
Rabbit anti-Oryza sativa subsp. japonica (Rice) NIP2-1 Polyclonal antibody
Uniprot No.
Target Names
NIP2-1
Alternative Names
NIP2-1; LSI1; SIIT1; Os02g0745100; LOC_Os02g51110; OJ1118_G04.16; OJ1734_E02.43; OsJ_008085; Aquaporin NIP2-1; Low silicon protein 1; NOD26-like intrinsic protein 2-1; OsNIP2;1; Silicon influx transporter LSI1
Raised in
Rabbit
Species Reactivity
Oryza sativa subsp. japonica (Rice)
Immunogen
Recombinant Oryza sativa subsp. japonica (Rice) NIP2-1 protein
Immunogen Species
Oryza sativa subsp. japonica (Rice)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Silicon influx transporter responsible for silicon transport from the external solution to the root cells. Is coupled with the silicon efflux transporter LSI2 in both exodermal and endodermal root cells for an efficient silicon transport across the cells into the stele. Silicon is beneficial to plant growth and helps plants to overcome abiotic and biotic stresses by preventing lodging (falling over) and increasing resistance to pests and diseases, as well as other stresses. Is coupled with LSI2 transporter in roots for efficient uptake of arsenite, which is further dispatched in shoots and grains. Mediates uptake of methylated arsenic species in roots.
Gene References into Functions
  1. Root elongation was decreased with increasing external Cd concentrations in both wild types and Lsi1 and Lsi2 mutants, but Si did not show an alleviative effect on Cd toxicity in all lines. By contrast, the Cd concentration in both the shoots and roots was decreased by Si in the WTs, but not in the mutants. PMID: 29045756
  2. Lsi1 plays a key role in rice, increasing the absorption and accumulation of Silicon, then alters antioxidant activities, and improves morphological properties. PMID: 28191468
  3. Low silicon rice 1 (Lsi1) gene controls silicon accumulation in rice and is constitutively expressed in the roots. [lsi1] PMID: 16572174
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
MIP/aquaporin (TC 1.A.8) family, NIP (TC 1.A.8.12) subfamily
Tissue Specificity
Mainly expressed in the roots. In roots, it localizes in the main and lateral roots, but not in root hairs. Within a root, it localizes on the plasma membrane of the distal side of both exodermis and endodermis, where casparian strips exist (at protein le
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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