NIP5-1 Antibody

Code CSB-PA890454XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NIP5-1 Polyclonal antibody
Uniprot No.
Target Names
NIP5-1
Alternative Names
NIP5-1; NLM6; At4g10380; F24G24.180; Probable aquaporin NIP5-1; NOD26-like intrinsic protein 5-1; AtNIP5;1; Nodulin-26-like major intrinsic protein 6; NodLikeMip6; Protein NLM6
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NIP5-1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Boric acid transporter. Low water transport activity. Plays an important role as plasma membrane boric acid channel for the boron uptake required for plant growth and development under boron limitation.
Gene References into Functions
  1. The results demonstrate that the polar localization of NIP5;1 is maintained by clathrin-mediated endocytosis, is dependent on phosphorylation in the TPG repeat, and is necessary for the efficient transport of boron in roots. PMID: 28341806
  2. The direct root uptake of urea was increased under boron deficiency, in both the high and low affinity range. This activity was entirely mediated by the NIP5;1 channel. PMID: 25957355
  3. Rough mapping and whole-genome sequencing identified the mutant with altered localization of NIP5;1 as an allele of UDP-glucose 4-epimerase 4. PMID: 24343997
  4. The 5' UTR controls boron-dependent NIP5;1 mRNA degradation and that NIP5;1 mRNA degradation is important for plant acclimation to high-boron conditions. PMID: 21908722
  5. The residue at the H5 helix position of the aromatic/arginine filter of both OsLsi1 and AtNIP5;1 plays a key role in the permeability to silicon and boron, but there is a relatively low selectivity for arsenite. PMID: 21586431
  6. NIP5;1 functions as a boric acid channel for Boron uptake and is crucial for plant growth under Boron limitation in Arabidopsis. PMID: 16679457
  7. Enhanced expression of NIP5;1 resulted in improved root elongation under boron-limiting conditions. PMID: 19017629
  8. Nodulin26-like Intrinsic Proteins (NIPs) AtNIP5;1 and AtNIP6;1 from A. thaliana, OsNIP2;1 and OsNIP3;2 from O. sativa, and LjNIP5;1 and LjNIP6;1 from Lotus japonicus are bi-directional As(III) channels. PMID: 18544156

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
MIP/aquaporin (TC 1.A.8) family, NIP (TC 1.A.8.12) subfamily
Tissue Specificity
Expressed in rosette leaves.
Database Links

KEGG: ath:AT4G10380

STRING: 3702.AT4G10380.1

UniGene: At.33633

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