Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Nipbl Polyclonal antibody

Uniprot No.

Q6KCD5

Target Names

Nipbl

Alternative Names

Nipbl antibody; Scc2 antibody; Nipped-B-like protein antibody; Delangin homolog antibody; SCC2 homolog antibody

Raised in

Rabbit

Species Reactivity

Mus musculus (Mouse)

Immunogen

Recombinant Mus musculus (Mouse) Nipbl protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Plays an important role in the loading of the cohesin complex on to DNA. Forms a heterodimeric complex (also known as cohesin loading complex) with MAU2/SCC4 which mediates the loading of the cohesin complex onto chromatin. Plays a role in cohesin loading at sites of DNA damage. Its recruitment to double-strand breaks (DSBs) sites occurs in a CBX3-, RNF8- and RNF168-dependent manner whereas its recruitment to UV irradiation-induced DNA damage sites occurs in a ATM-, ATR-, RNF8- and RNF168-dependent manner. Along with ZNF609, promotes cortical neuron migration during brain development by regulating the transcription of crucial genes in this process. Preferentially binds promoters containing paused RNA polymerase II. Up-regulates the expression of SEMA3A, NRP1, PLXND1 and GABBR2 genes, among others.

Gene References into Functions

Depletion of Zfp609 or Nipbl from cortical neural progenitors in vivo is detrimental to neuronal migration. Zfp609 and Nipbl overlap at genomic binding sites independently of cohesin and regulate genes that control cortical neuron migration. PMID: 28041881
Nipbl transgenic mice display large atrial septal defects. PMID: 27606604
This study provides insight into the molecular pathology of Cornelia de Lange syndrome by establishing a relationship between NIPBL and HDAC8 mutations and PKR activation. PMID: 26725122
expression analysis of Smc1a and Nipbl in developing mouse embryos reveals a specific pattern in the hindbrain PMID: 26206533
Nipbl and mediator cooperatively regulate gene expression to control limb development. PMID: 25255084
Nipbl+/- mutants are growth-retarded and exhibit various skeletal and craniofacial malformations. PMID: 24700590
In spermatocytes, Nipbl/Mau2 then relocalises to chromocenters, whereas in oocytes it remains bound to chromosomal axes throughout prophase to dictyate arrest. PMID: 24287868
Reduction of Nipbl is associated with Cornelia de Lange Syndrome. PMID: 23920377
Mice heterozygous for a gene-trap mutation in Nipbl were produced and exhibited defects characteristic of Cornelia de Lange Syndrome. PMID: 19763162

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Subcellular Location

Nucleus. Chromosome.

Protein Families

SCC2/Nipped-B family

Tissue Specificity

Spermatocytes and oocytes (at protein level).

Database Links

KEGG: mmu:71175

STRING: 10090.ENSMUSP00000059385

UniGene: Mm.240329

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Nipbl Antibody

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