nlpE Antibody

Datasheet

Code	CSB-PA331212XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) nlpE Polyclonal antibody

Uniprot No.

P40710

Target Names

nlpE

Alternative Names

nlpE antibody; cutF antibody; b0192 antibody; JW0188 antibody; Lipoprotein NlpE antibody; Copper homeostasis protein CutF antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) nlpE protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Involved in copper homeostasis, could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. Required for efficient binding of stationary phase cells to hydrophobic surfaces, part of the process of biofilm formation. Functions during envelope stress responses; when overproduced induces degP through the activation of the two-component envelope stress response system CpxA/CpxR. DegP induction seems to require membrane anchoring of this protein. Structural changes and/or interaction of the CXXC motif with its environment may lead to activation of the Cpx stress response.

Gene References into Functions

Sensing of adhesion signals via NlpE is important for regulation of the expression of the type III secretion system and flagella in enterohemorrhagic Escherichia coli during infection PMID: 26644384
The nonlipidated Cys1Ala mutant of NlpE with a C-terminal His tag from Escherichia coli was constructed, overexpressed and purified. The tetragonal crystals diffracted to 2.8 A resolution and belong to space group P4(3)2(1)2. PMID: 17142903

Subcellular Location

Cell outer membrane; Lipid-anchor.

Database Links

KEGG: ecj:JW0188

STRING: 316385.ECDH10B_0173

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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nlpE Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background