norR Antibody

Code CSB-PA327626XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) norR Polyclonal antibody
Uniprot No.
Target Names
norR
Alternative Names
norR antibody; ygaA antibody; b2709 antibody; JW5843 antibody; Anaerobic nitric oxide reductase transcription regulator NorR antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) norR protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for the expression of anaerobic nitric oxide (NO) reductase, acts as a transcriptional activator for at least the norVW operon. Activation also requires sigma-54. Not required for induction of the aerobic NO-detoxifying enzyme NO dioxygenase. Binds to the promoter region of norVW, to a consensus target sequence, GT-(N7)-AC, which is highly conserved among proteobacteria.
Gene References into Functions
  1. These observations suggest a novel mechanism for negative regulation in bacterial enhancer binding proteins such as NorR in which the GAF domain targets the sigma(54)-interaction surface to prevent access of the AAA+ domain to the sigma factor. PMID: 20624215
  2. the regulatory domain of NorR contains a mononuclear non-haem iron centre, which reversibly binds NO; binding of NO stimulates the ATPase activity of NorR, enabling the activation of transcription by RNA polymerase PMID: 16193057
  3. Analysis of the interaction of NO with the nonheme iron center and determination of the NO-binding affinity constant. PMID: 18433632
Database Links
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