NOSIP Antibody

Code CSB-PA897477XA01HU
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) NOSIP Polyclonal antibody
Uniprot No.
Target Names
NOSIP
Alternative Names
NOSIP antibody; CGI-25 antibody; Nitric oxide synthase-interacting protein antibody; E3 ubiquitin-protein ligase NOSIP antibody; EC 2.3.2.27 antibody; RING-type E3 ubiquitin transferase NOSIP antibody; eNOS-interacting protein antibody
Raised in
Rabbit
Species Reactivity
Homo sapiens (Human)
Immunogen
Recombinant Homo sapiens (Human) NOSIP protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB. Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity.
Gene References into Functions
  1. While NOSIP expression was increased in Hirschsprung's disease (HSCR) vs. non- HSCR patients, no significant difference was observed in patients with Hirschsprung's associated enterocolitis. The increased expression of NOSIP in the aganglionic and ganglionic bowel of HSCR may contribute to the development of enterocolitis by inhibiting local nitric oxide production in patients with Hirschsprung's disease. PMID: 28196663
  2. Endogenous NOSIP reduces the enzymatic capacity of eNOS, specifically in the G(2) phase of the cell cycle by targeting eNOS to the actin cytoskeleton. PMID: 16135813
  3. endothelial nitric oxide synthase (eNOS) interacting protein (NOSIP), modulator of eNOS activity by alteration of eNOS subcellular localisation; high expression levels in endothelial cells and vascularized tissue. PMID: 11149895
Subcellular Location
Cytoplasm. Nucleus. Note=Translocates from nucleus to cytoplasm in the G2 phase of the cell cycle (PubMed:16135813).
Protein Families
NOSIP family
Tissue Specificity
Expressed in heart, brain and lung. Present in endothelial cells (at protein level).
Database Links

HGNC: 17946

OMIM: 616759

KEGG: hsa:51070

STRING: 9606.ENSP00000375726

UniGene: Hs.7236

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