NPC4 Antibody

Code CSB-PA138325XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NPC4 Polyclonal antibody
Uniprot No.
Target Names
NPC4
Alternative Names
NPC4 antibody; At3g03530 antibody; T21P5.5 antibody; Non-specific phospholipase C4 antibody; EC 3.1.4.3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NPC4 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Non-specific phospholipase C (PLC) which assumes major PLC activity during inorganic phosphate starvation. Substrate preference is phosphatidylcholine (PC), but can also hydrolyze phosphatidylethanolamine (PE) with lower efficiency. Has no activity toward phosphatidic acid (PA). Plays an important role in the supply of both inorganic phosphate and diacylglycerol from membrane-localized phospholipids during phosphate deprivation. May be required for lipid-derived signaling molecules that positively modulate abscisic acid (ABA) response and promote plant tolerance to drought and salt stresses. May be involved in brassinolide-mediated signaling in root development.
Gene References into Functions
  1. Under salt stress npc4 plants had shorter roots, lower fresh weight, and reduced seed germination. Expression levels of abscisic acid-related genes ABI1, ABI2, RAB18, PP2CA, and SOT12 were substantially reduced in salt-treated npc4 plants. PMID: 21525137
  2. NPC4 plays an important role in plant response to abscisic acid, drought, and high salinity. PMID: 20699393
  3. NPC4 plays an important role in the supply of both inorganic phosphate and diacylglycerol from membrane-localized phospholipids [NPC4] PMID: 15618226
Subcellular Location
Cell membrane; Peripheral membrane protein.
Protein Families
Bacterial phospholipase C family
Tissue Specificity
Expressed in root tips, cotyledons, on leaf margins, stems, young anthers and funiculus.
Database Links

KEGG: ath:AT3G03530

STRING: 3702.AT3G03530.1

UniGene: At.18482

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