cobB Antibody

Code CSB-PA304314XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) cobB Polyclonal antibody
Uniprot No.
Target Names
cobB
Alternative Names
cobB antibody; ycfY antibody; b1120 antibody; JW1106 antibody; NAD-dependent protein deacylase antibody; EC 2.3.1.286 antibody; Regulatory protein SIR2 homolog antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) cobB protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form. Activates the enzyme acetyl-CoA synthetase by deacetylating 'Lys-609' in the inactive, acetylated form of the enzyme. May also modulate the activity of other propionyl-adenosine monophosphate (AMP)-forming enzymes.
Gene References into Functions
  1. discovered that CobB acts as a lipoamidase; results indicate that CobB has the ability to regulate pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase activity under different growth conditions PMID: 28900027
  2. CobB can reverse acetylation regardless of acetyl donor or acetylation mechanism. PMID: 25417765
  3. CobB is a bifunctional enzyme with lysine desuccinylation and deacetylation activities. PMID: 24176774
  4. CobB play crucial roles in the transcription regulation of the periplasmic stress-responsive promoter cpxP when cells of Escherichia coli grow in the presence of glucose, an environment that induces protein acetylation PMID: 21696463
  5. Results suggest that CobB regulates E. coli chemotaxis by deacetylating CheY. PMID: 20345663

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Subcellular Location
Cytoplasm.
Protein Families
Sirtuin family, Class III subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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