Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) NPFR Polyclonal antibody

Uniprot No.

Q9VNM1

Target Names

NPFR

Alternative Names

NPFR antibody; NPFR1 antibody; CG1147 antibody; Neuropeptide F receptor antibody; DmNPFR1 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) NPFR protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Receptor for NPF. Integral part of the sensory system that mediates food signaling, providing the neural basis for the regulation of food response; coordinates larval foraging and social behavior changes during development. Required in dopaminergic (DA) neurons that innervate the mushroom body for satiety to suppress appetitive memory performance; a key factor in the internal state of hunger in the brain. NPF neurons coordinately modulate diverse sensory and motor neurons important for feeding, flight, and locomotion. NPF/NPFR pathway exerts its suppressive effect on larval aversion to diverse stressful stimuli (chemical stress and noxious heat) through attenuation of TRP channel-induced neuronal excitation. NPF neural signaling system plays a physiological role in acute modulation of alcohol sensitivity in adults, rather than a general response to intoxication by sedative agents. Activation and inhibition of the NPF system reduces and enhances ethanol preference, respectively. Sexual experience, the NPF system activity and ethanol consumption are all linked; sexual deprivation is a major contributor to enhanced ethanol preference.

Gene References into Functions

Male-specific sex-dependent regulatory function of sleep is controlled by NPF and NPFR1 expression in the brain. PMID: 24040211
Distribution of short neuropeptide F and its receptor in neuronal circuits related to feeding in larval Drosophila. PMID: 23760890
in males, mating increased, whereas sexual deprivation reduced, NPF levels; propose that activity of the NPF-NPF receptor axis represents the state of the fly reward system and modifies behavior accordingly PMID: 22422983
Expression of sNPF and its receptor (sNPFR1) in Or42b neurons is necessary for starvation-induced food-search behavior. PMID: 21458672
expressed in CNS and midgut; a functional receptor for Drosophila neuropeptide F (DmNPF) PMID: 11897397
NPFR requires an Arg residue at position 4 from the amidated C-terminus for ativation by short NPFs PMID: 12372405
Results identify a Drosophila signaling system, comprising neurons expressing neuropeptide F and its receptor, NPFR1, that acutely mediates sensitivity to ethanol sedation. PMID: 15677721

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Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family

Tissue Specificity

Expressed in midgut, brain lobes and ventral nerve cord of larvae. In adults, expressed in a pair of dorsolateral neurons in the protocerebrum, and the central complex and a small number of neurons in the subesophageal ganglion (at protein level). Express

Database Links

KEGG: dme:Dmel_CG1147

STRING: 7227.FBpp0300637

UniGene: Dm.2483

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NPFR Antibody

Product Details

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Target Background