npp-5 Antibody

Code CSB-PA117223XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans npp-5 Polyclonal antibody
Uniprot No.
Target Names
npp-5
Alternative Names
npp-5 antibody; F07A11.3 antibody; Nuclear pore complex protein 5 antibody; Nuclear pore complex protein Nup107 antibody; Nucleoporin npp-5 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans npp-5 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in kinetochore assembly and chromosome segregation during embryonic mitosis. Required for the localization of the NDC80 complex member him-10, the chromosomal passenger complex component air-2 and nuclear pore complex proteins npp-23 and npp-15 to kinetochores during metaphase. Required for npp-23 localization to the nuclear envelope during interphase. Recruits mdf-1, a component of the spindle assembly checkpoint, to the nuclear envelope. Appears dispensable for the assembly of the nuclear pore complex and for nuclear protein import.
Gene References into Functions
  1. NPP-5 interacts genetically and physically with SAC protein MAD1/MDF-1, whose nuclear envelope accumulation requires NPP-5. PMID: 22238360
Subcellular Location
Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Nucleus membrane; Peripheral membrane protein.
Protein Families
Nucleoporin Nup84/Nup107 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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