Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NPR5 Polyclonal antibody

Uniprot No.

Q9ZVC2

Target Names

NPR5

Alternative Names

NPR5 antibody; BOP2 antibody; At2g41370 antibody; F13H10.8 antibody; Regulatory protein NPR5 antibody; BTB/POZ domain-containing protein NPR5 antibody; Protein BLADE ON PETIOLE 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana NPR5 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Acts redundantly with BOP2. BOP1/2 promote leaf and floral meristem fate and determinacy in a pathway targeting AP1 and AGL24. BOP1/2 act as transcriptional co-regulators through direct interaction with TGA factors, including PAN, a direct regulator of AP1. Controls lateral organ fate through positive regulation of adaxial-abaxial polarity genes ATHB-14/PHB, YAB1/FIL and YAB3, and through positive regulation of LOB domain-containing genes LOB, LBD6/AS2 and LBD36. Promotes and maintains a developmentally determinate state in leaf cells through the negative regulation of JAG, JGL and class I KNOX genes. Is also involved in nectary development, formation of normal abscission zones and suppression of bract formation.

Gene References into Functions

This shows that BOP proteins act as substrate adaptors in a CUL3(BOP1/BOP2) E3 ubiquitin ligase complex, targeting PIF4 proteins for ubiquitination and subsequent degradation. PMID: 28826468
HAN physically interacts with JAG, and directly stimulates the expression of JAG and BOP2 to regulate floral organ development PMID: 26390296
Only the reduction of both bop1 and bop2 expression affects resistance triggered by methyl jasmonate. PMID: 23116333
FYF controls floral senescence/abscission by repressing ethylene responses and regulating the expression of BOP2 and IDA in Arabidopsis. PMID: 21689171
BOP1/2 gain-of-function in stems promotes expression of the boundary gene KNOTTED1-LIKE FROM ARABIDOPSIS THALIANA6 (KNAT6) and ARABIDOPSIS THALIANA HOMEOBOX GENE1 (ATH1). PMID: 22751300
Data reveal an important supporting role for BOP1/2 in remodeling shoot architecture during the floral transition. PMID: 20626659
BOP1 and BOP2 act in cells adjacent to the lateral organ boundary to repress genes that confer meristem cell fate and induce genes that promote lateral organ fate and polarity. PMID: 17601823
BOP1/BOP2 activity is required for multiple cell differentiation events in the proximal regions of inflorescence lateral organs. PMID: 18339677

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Subcellular Location

Cytoplasm. Nucleus.

Tissue Specificity

Highly expressed in young floral meristem. Predominantly expressed in the boundary between floral meristem (FM) and sepal primordia.

Database Links

KEGG: ath:AT2G41370

STRING: 3702.AT2G41370.1

UniGene: At.43967

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NPR5 Antibody

Product Details

Customer Reviews and Q&A

Target Background