npas4l Antibody

Code CSB-PA438232XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) npas4l Polyclonal antibody
Uniprot No.
Target Names
npas4l
Alternative Names
npas4l antibody; clo antibody; Neuronal PAS domain-containing protein 4-like antibody; Protein cloche antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) npas4l protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor specifically expressed in endothelial and hematopoietic precursor cells that acts as a key regulator of the endothelial differentiation cascade. Acts as an early-response transcription factor that regulates the expression of early regulators of endothelial and haematopoietic differentiation, such as etv2 and tal1.
Gene References into Functions
  1. The endothelial cells in clo(172) mutant embryos, although initially developed, failed to form the functional vascular system eventually.[Cloche] PMID: 22132109
  2. the disorganized retinal phenotype of clo-/- embryos is consistent with a neural and glial developmental patterning role for the early ocular vasculature that is independent of its eventual function in gas exchange PMID: 26283463
  3. identifying master regulator of endothelial and haematopoietic fate, cloche, enhances our understanding of early mesoderm diversification and may lead to improved protocols for the generation of endothelial and haematopoietic cells in vivo and in vitro PMID: 27411634
  4. cloche(172) gene was mapped on the telomere of zebrafish 13 chromosome. Numerous red blood cells were observed in the cloche(172) mutant. PMID: 20335109
Subcellular Location
Nucleus.
Tissue Specificity
Specifically expressed in endothelial and hematopoietic precursor cells.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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