wrbA Antibody

Code CSB-PA359132XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) wrbA Polyclonal antibody
Uniprot No.
Target Names
wrbA
Alternative Names
wrbA antibody; b1004 antibody; JW0989 antibody; NAD(P)H dehydrogenase antibody; quinone antibody; EC 1.6.5.2 antibody; Flavoprotein WrbA antibody; NAD(P)H:quinone oxidoreductase antibody; NQO antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) wrbA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
It seems to function in response to environmental stress when various electron transfer chains are affected or when the environment is highly oxidizing. It reduces quinones to the hydroquinone state to prevent interaction of the semiquinone with O2 and production of superoxide. It prefers NADH over NADPH.
Gene References into Functions
  1. FMN binding has a dramatic effect on WrbA thermal stability, shifting the Tm by approximately 40 degrees C. PMID: 17209564
  2. Structural comparison of WrbA, flavodoxin, and Nqo1 indicates how the twisted open-sheet fold of flavodoxins is elaborated to form multimers that extend catalytic function from one-electron transfer between protein partners. PMID: 17893367
  3. Topologically non-equivalent residues undergo remarkably similar local structural changes upon Flavin Mononucleotide binding to WrbA or to flavodoxin, despite differences in multimeric organization and residue types at the binding sites. PMID: 19665595
Protein Families
WrbA family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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