NRAMP4 Antibody

Code CSB-PA884305XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NRAMP4 Polyclonal antibody
Uniprot No.
Target Names
NRAMP4
Alternative Names
NRAMP4 antibody; At5g67330 antibody; K8K14.5 antibody; Metal transporter Nramp4 antibody; AtNramp4 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NRAMP4 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Vacuolar metal transporter involved in intracellular metal homeostasis. Can transport iron (Fe), manganese (Mn) and cadmium (Cd). Regulates metal accumulation under Fe starvation. Acts redundantly with NRAMP3 to mobilize vacuolar Fe and provide sufficient Fe during seed germination. In association with NRAMP3, required for optimal growth and photosynthesis under Mn deficiency. Exports Mn from vacuoles in leaf mesophyll cells, making Mn available for functional photosystem II in chloroplasts.
Gene References into Functions
  1. Mobilization of vacuolar iron (Fe) stores by AtNRAMP4 is crucial to support Arabidopsis early development until efficient systems for Fe acquisition from the soil take over. PMID: 16270029
Subcellular Location
Vacuole membrane; Multi-pass membrane protein.
Protein Families
NRAMP (TC 2.A.55) family
Tissue Specificity
Expressed in vascular tissues.
Database Links

KEGG: ath:AT5G67330

STRING: 3702.AT5G67330.1

UniGene: At.19993

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