nrfA Antibody

Code CSB-PA989284XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) nrfA Polyclonal antibody
Uniprot No.
Target Names
nrfA
Alternative Names
nrfA antibody; b4070 antibody; JW4031 antibody; Cytochrome c-552 antibody; EC 1.7.2.2 antibody; Ammonia-forming cytochrome c nitrite reductase antibody; Cytochrome c nitrite reductase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) nrfA protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the reduction of nitrite to ammonia, consuming six electrons in the process. Has very low activity toward hydroxylamine. Has even lower activity toward sulfite. Sulfite reductase activity is maximal at neutral pH.
Gene References into Functions
  1. Kinetic and thermodynamic resolution of the interactions between sulfite and the pentahaem cytochrome NrfA from Escherichia coli PMID: 20629638
  2. Activation of the Escherichia coli K-12 nrf promoter is downregulated by the nitric oxide-sensitive NsrR protein together with the nucleoid-associated protein IHF. PMID: 20472787
  3. Protein-protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2-NrfB2 heterotetrameric complex. PMID: 17521287
  4. Method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies. PMID: 18433623
  5. Spectroelectrochemical studies of E. coli NrfA have provided unprecedented resolution of its thermodynamic properties. PMID: 18549208

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Subcellular Location
Periplasm.
Protein Families
Cytochrome c-552 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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