Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NRPB1 Polyclonal antibody

Uniprot No.

P18616

Target Names

NRPB1

Alternative Names

NRPB1 antibody; RPB1 antibody; RPB205 antibody; RPII antibody; At4g35800 antibody; F4B14.70 antibody; DNA-directed RNA polymerase II subunit RPB1 antibody; DNA polymerase II subunit B1 antibody; EC 2.7.7.6 antibody; DNA-directed RNA polymerase II largest subunit antibody; DNA-directed RNA polymerase II subunit 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana NRPB1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. NRPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from NRPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (NRPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing.

Gene References into Functions

RPB1 is required for shoot regeneration. PMID: 29625106
The mutation nrpb1-A325V in the largest subunit of RNA polymerase II suppresses compromised growth of Arabidopsis plants deficient in a function of the general transcription factor IIF PMID: 27862530
Data indicate that intron-mediated enhancement (IME) of the GGT1 5'UTR intron is controlled on the transcriptional level, and that the abundance of RNA polymerase II binding to the intron-less construct is reduced. PMID: 27418588

Subcellular Location

Nucleus.

Protein Families

RNA polymerase beta' chain family

Database Links

KEGG: ath:AT4G35800

STRING: 3702.AT4G35800.1

UniGene: At.23499

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NRPB1 Antibody

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