NRPB3 Antibody

Code CSB-PA655796XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NRPB3 Polyclonal antibody
Uniprot No.
Target Names
NRPB3
Alternative Names
NRPB3 antibody; NRPD3 antibody; NRPE3A antibody; RPB36A antibody; At2g15430 antibody; F26H6.5 antibody; DNA-directed RNA polymerases II antibody; IV and V subunit 3 antibody; DNA-directed RNA polymerase II 36 kDa polypeptide A antibody; DNA-directed RNA polymerase II subunit RPB3-A antibody; RNA polymerase II subunit 3-A antibody; RNA polymerase II subunit B3-A antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NRPB3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. NRPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. Component of RNA polymerases IV and V which mediate short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation-dependent (RdDM) transcriptional gene silencing (TGS) of endogenous repeated sequences, including transposable elements.
Gene References into Functions
  1. Protein-protein interaction assays showed that NRPB3 directly interacts with two basic helix-loop-helix transcription factors, FAMA and INDUCER OF CBF EXPRESSION1, indicating that NRPB3 serves as an acceptor for signals from transcription factors involved in stomatal development. PMID: 26989174
Subcellular Location
Nucleus.
Protein Families
Archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family
Database Links

KEGG: ath:AT2G15430

STRING: 3702.AT2G15430.1

UniGene: At.27427

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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