NRPB7 Antibody

Code CSB-PA018334XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NRPB7 Polyclonal antibody
Uniprot No.
Target Names
NRPB7
Alternative Names
NRPB7 antibody; RPB19 antibody; At5g59180 antibody; MNC17.9 antibody; DNA-directed RNA polymerase II subunit 7 antibody; RNA polymerase II subunit B7 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NRPB7 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. NRPB7 is part of a subcomplex with NRPB4 that binds to a pocket formed by NRPB1, NRPB2 and NRPB6 at the base of the clamp element. The NRBP4-NRPB7 subcomplex seems to lock the clamp via NRPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The NRPB4-NRPB7 subcomplex binds single-stranded DNA and RNA.
Subcellular Location
Nucleus.
Protein Families
Eukaryotic RPB7/RPC8 RNA polymerase subunit family
Database Links

KEGG: ath:AT5G59180

STRING: 3702.AT5G59180.1

UniGene: At.29242

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