Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NRPD1 Polyclonal antibody

Uniprot No.

Q9LQ02

Target Names

NRPD1

Alternative Names

NRPD1 antibody; NRPD1a antibody; RMD3 antibody; RPD1 antibody; SDE4 antibody; SMD2 antibody; At1g63020 antibody; F16M19.19 antibody; F16P17.19 antibody; DNA-directed RNA polymerase IV subunit 1 antibody; DNA-directed RNA polymerase D subunit 1 antibody; AtNRPD1a antibody; Nuclear RNA polymerase D 1a antibody; EC 2.7.7.6 antibody; Protein RNA-DIRECTED DNA METHYLATION DEFECTIVE 3 antibody; Protein SILENCING DEFECTIVE 4 antibody; Protein SILENCING MOVEMENT DEFICIENT 2 antibody; RNA polymerase IV subunit 1a antibody; POL IV 1a antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana NRPD1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase IV which mediates 24-nt short-interfering RNAs (siRNA) accumulation. Implicated in siRNA-directed heterochromatin formation through the action of DCL3 and AGO4, and subsequent DNA methylation-dependent silencing of targeted sequences. Essential component of a self-reinforcing loop coupling de novo DNA methylation to siRNA production. Required for intercellular but not intracellular RNA interference (RNAi) leading to systemic post-transcriptional gene silencing. Involved in the maintenance of post-transcriptional RNA silencing.

Gene References into Functions

Pol IV-dependent (p4-)siRNAs are initially expressed in the maternal gametophyte and uniparentally expressed from maternal chromosomes in developing endosperm. PMID: 20074090
results show that RNA polymerase IV (Pol IV, subunits NRPD1a and NRPD2) silences certain transposons and repetitive DNA in a short interfering RNA pathway involving RNA-dependent RNA polymerase 2 and Dicer-like 3(DCL3) [NRPD1a] PMID: 15692015
RNA Pol IV helps produce siRNAs that target de novo cytosine methylation events required for facultative heterochromatin formation and higher-order heterochromatin associations.[NRPD1a; RNA Pol IV] PMID: 15766525
RNA polymerase IV has a role in plant small RNA metabolism [RNAP IV] PMID: 17360559
NRPD1a is required for reception of long-distance mRNA silencing. PMID: 17785412
Mutants defective for the NRPD1a and NRPD1b alternative largest subunits of polymerase IV were tested for their ability to undergo RNA silencing and mantenance of transcription and post-transcriptional silencing PMID: 18433438

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Subcellular Location

Nucleus. Note=Follows a punctate localization pattern.

Protein Families

RNA polymerase beta' chain family

Tissue Specificity

Mostly expressed in flowers, and, to a lower extent, in leaves.

Database Links

KEGG: ath:AT1G63020

STRING: 3702.AT1G63020.1

UniGene: At.36192

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NRPD1 Antibody

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