Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NRPE1 Polyclonal antibody

Uniprot No.

Q5D869

Target Names

NRPE1

Alternative Names

NRPE1 antibody; DMS5 antibody; DRD3 antibody; NRPD1b antibody; RMD1 antibody; RPE1 antibody; At2g40030 antibody; T28M21.19 antibody; DNA-directed RNA polymerase V subunit 1 antibody; DNA-directed RNA polymerase D subunit 1b antibody; AtNRPD1b antibody; Nuclear RNA polymerase D 1b antibody; DNA-directed RNA polymerase E subunit 1 antibody; Nuclear RNA polymerase E 1 antibody; EC 2.7.7.6 antibody; Protein DEFECTIVE IN MERISTEM SILENCING 5 antibody; Protein DEFECTIVE IN RNA-DIRECTED DNA METHYLATION 3 antibody; Protein RNA-DIRECTED DNA METHYLATION DEFECTIVE 1 antibody; RNA polymerase IV subunit 1 antibody; POL IV 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana NRPE1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase V involved in RNA-directed DNA methylation-dependent (RdDM) silencing of endogenous repeated sequences, including transposable elements. Also required for full erasure of methylation when the RNA trigger is withdrawn. Seems also involved in the synthesis of short-interfering RNAs (siRNA). Essential component of a self-reinforcing loop coupling de novo DNA methylation to siRNA production. Involved in the maintenance of post-transcriptional RNA silencing.

Gene References into Functions

DRD1-Pol V-dependent de novo methylation may play a role in restraining the inappropriate silencing of active protein-coding genes in plants. [NRPE1] PMID: 21771120
Mutants defective for the NRPD1a and NRPD1b alternative largest subunits of polymerase IV were tested for their ability to undergo RNA silencing and mantenance of transcription and post-transcriptional silencing. PMID: 18433438

Subcellular Location

Nucleus, nucleolus. Note=Clustered within heterochromatic regions and colocalized with RDR2, DCL3, NRPD4, AGO4, and siRNAs within the nucleolus. Recruited to chromatin via its interaction with SUVH2.

Protein Families

RNA polymerase beta' chain family

Tissue Specificity

Mostly expressed in flowers, and, to a lower extent, in leaves. Present in sperm cells.

Database Links

KEGG: ath:AT2G40030

STRING: 3702.AT2G40030.1

UniGene: At.37106

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA710698XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

NRPE1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background