NRT2.5 Antibody

Code CSB-PA864808XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NRT2.5 Polyclonal antibody
Uniprot No.
Target Names
NRT2.5
Alternative Names
NRT2.5 antibody; At1g12940 antibody; F13K23.20 antibody; High affinity nitrate transporter 2.5 antibody; AtNRT2:5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NRT2.5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Nitrate transporter involved in the constitutive high-affinity transport system (cHATS) under long-term N starvation conditions. Predominantly expressed in roots of nitrate-deprived plants as a 150 kDa molecular complex with NRT3.1 representing the major contributor to cHATS influx. The principal role of this cHATS is to enable roots previously deprived of nitrate to absorb this ion and initiate induction of nitrate-inducible genes. Not involved in transfer of nitrate from roots to shoots. Contributes to phloem loading of nitrate in shoots during N starvation, but not required for growth and nitrate uptake in young plants. Required for the nitrate uptake-independent plant growth promotion and lateral root response to the rhizospheric Phyllobacterium. Might be involved in the transfer of nitrate from stored pools to cytoplasm.
Gene References into Functions
  1. AtNRT2.5 is predominantly expressed in roots of nitrate-deprived WT plants as a 150 kDa molecular complex with AtNAR2.1. Estimates of the kinetic properties of the NRT2.5 transporter reveal that its low Km for nitrate makes this transporter ideally suited to detect and respond to trace quantities of nitrate in the root environment. [AtNRT2.5] PMID: 25474587
  2. Growth analyses of multiple mutants between NRT2.1, NRT2.2, NRT2.4, and NRT2.5 revealed that NRT2.5 is required to support growth of nitrogen-starved adult plants by ensuring the efficient uptake of nitrate collectively with NRT2.1, NRT2.2 and NRT2.4 PMID: 25065551
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Major facilitator superfamily, Nitrate/nitrite porter (TC 2.A.1.8) family
Tissue Specificity
Expressed in roots, shoots and seeds. Expressed in leaves. Expressed in root hair zone of the primary root and the lateral roots, but not in the lateral root tip or in older parts of the roots. Detected mainly in the epidermis and the cortex. Expressed in
Database Links

KEGG: ath:AT1G12940

STRING: 3702.AT1G12940.1

UniGene: At.42054

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