NRT3.1 Antibody

Code CSB-PA872221XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NRT3.1 Polyclonal antibody
Uniprot No.
Target Names
NRT3.1
Alternative Names
NRT3.1; NAR2.1; NAR2.2; WR3; At5g50200; K6A12.6; High-affinity nitrate transporter 3.1; Protein WOUND-RESPONSIVE 3
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NRT3.1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as a dual component transporter with NTR2.1. Required for high-affinity nitrate transport. Acts as a repressor of lateral root initiation. May be involved in targeting NRT2 proteins to the plasma membrane.
Gene References into Functions
  1. AtNRT2.5 is predominantly expressed in roots of nitrate-deprived WT plants as a 150 kDa molecular complex with AtNAR2.1 which is the major contributor to constitutive high-affinity nitrate influx in Arabidopsis thaliana. [NAR2.1] PMID: 25474587
  2. Except for AtNRT2.7, all other NRT2 transporters interact strongly with AtNAR2.1. PMID: 22432443
  3. Data demonstrate that an intact two-component complex of AtNRT2.1 and AtNAR2.1 (AtNRT3.1) is localized in the plasma membrane. PMID: 20561257
  4. The constitutive high-affinity influx and nitrate-inducible high-affinity influx (but not the low-affinity influx) of higher plant roots require a functional AtNRT3 gene. [AtNRT3.1] PMID: 16415212
  5. The NRT3.1 gene encodes a key component of the HATS (high-affinity NO3 transport system) and repression of NRT2.1 and NRT3.1 by high nitrates is specifically mediated by the NRT1.1 transporter. [NRT3.1] PMID: 16998085
  6. AtNAR2.1 is essential for high-affinity transport system of nitrate in Arabidopsis.[NAR2.1] PMID: 17012411

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Subcellular Location
Cell membrane; Single-pass membrane protein.
Protein Families
NAR2 family
Tissue Specificity
Highly expressed in roots. Detected in shoots.
Database Links

KEGG: ath:AT5G50200

STRING: 3702.AT5G50200.1

UniGene: At.21028

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