MMS21 Antibody

Code CSB-PA810169XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) MMS21 Polyclonal antibody
Uniprot No.
Target Names
MMS21
Alternative Names
MMS21 antibody; HPY2 antibody; At3g15150 antibody; F4B12.6 antibody; E3 SUMO-protein ligase MMS21 antibody; EC 2.3.2.- antibody; E3 SUMO-protein transferase MMS21 antibody; MMS21 homolog antibody; AtMMS21 antibody; Protein HIGH PLOIDY 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana MMS21 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
E3 SUMO-protein ligase that modulates cell cycle progression and functions as a repressor of endocycle onset in meristems. May function downstream of the meristem patterning transcription factors PLETHORA 1 and 2 (PLT1 and PLT2) in root meristem development. Modulates the expression of the mitotic cyclins CYCB1-1 and CYCB1-2 and cyclin-dependent kinases CDKB1-1 and CDKB2-1 in root meristem. Involved in cytokinin signaling in root development.
Gene References into Functions
  1. Mutation of AtMMS21 increased sensitivity to DNA damaging agents and the constitutive activation of DNA damage response in mms21-1 may be mediated by ATM. PMID: 24795278
  2. AtMMS21 mediated SUMOylation may stabilize the expression and accumulation of meiotic proteins and affect gametophyte development. PMID: 24893774
  3. These data reveal that AtMMS21 acts in DNA double-strand breaks amelioration and stem cell niche maintenance during Arabidopsis root development. PMID: 23426194
  4. conclude that MMS21 plays a role in the drought stress response, likely through regulation of gene expression in an ABA-dependent pathway PMID: 23231763
  5. SIZ1 and HPY2 function independently and that their combined SUMOylation is essential for plant development PMID: 23056518
  6. Arabidopsis SUMO E3 ligase AtMMS21 is involved in apical root meristem development. [MMS21] PMID: 20592809
  7. AtMMS21 is an important regulator of cell proliferation and cytokinin signalling during root development. PMID: 19682286

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
NSE2 family
Database Links

KEGG: ath:AT3G15150

STRING: 3702.AT3G15150.1

UniGene: At.39098

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