MMS21 Antibody

Code CSB-PA336471XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MMS21 Polyclonal antibody
Uniprot No.
Target Names
MMS21
Alternative Names
MMS21 antibody; NSE2 antibody; YEL019C antibody; E3 SUMO-protein ligase MMS21 antibody; EC 2.3.2.- antibody; E3 SUMO-protein transferase MMS21 antibody; Methyl methanesulfonate-sensitivity protein 21 antibody; Non-structural maintenance of chromosome element 2 antibody; Non-SMC element 2 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MMS21 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts as an E3 ligase mediating SUMO/Smt3 attachment to SMC5 and YKU70. Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.
Gene References into Functions
  1. Mms21 prevents the accumulation of spontaneous DNA lesions that cause diverse gross chromosomal rearrangements. PMID: 29505562
  2. Data indicate that Snf1 Is the target of SUMO (E3) ligase Mms21. PMID: 24108357
  3. Deletion of E3 ligase domain of Mms21 led to decreased binding of Sir2p, Sir3p and Sir4 at subtelomeric chromatins and increased H3K4 tri-methylation at telomere-distal euchromatin regions. PMID: 23911609
  4. Small ubiquitin-related modifier ligase activity of Mms21 is required for maintenance of chromosome integrity during the unperturbed mitotic cell division cycle in Saccharomyces cerevisiae PMID: 21324902
  5. Mms21 requires the Siz/PIAS (protein inhibitor of activated signal transducer and activator of transcription) RING domain to function as a SUMO ligase. PMID: 15738391
  6. Molecular cloning proved pso10-1 to be a mutant allele of gene MMS21 that encodes a SUMO ligase involved in the sumoylation of several DNA repair proteins. PMID: 18437386

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
NSE2 family
Database Links

KEGG: sce:YEL019C

STRING: 4932.YEL019C

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