Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NTG1 Polyclonal antibody

Uniprot No.

P31378

Target Names

NTG1

Alternative Names

NTG1 antibody; OGG2 antibody; SCR1 antibody; YAL015C antibody; FUN33 antibody; Endonuclease III homolog 1 antibody; EC 3.2.2.- antibody; EC 4.2.99.18 antibody; Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase 1 antibody; DNA glycosylase/AP lyase 1 antibody; Endonuclease III-like glycosylase 1 antibody; Redoxyendonuclease 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) NTG1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines, but also purine-derived lesions, alkylation damage and cytosine photoproducts generated by UV irradiation as well as abasic sites. Has also 8-oxoguanine DNA glycosylase activity. The AP lyase can incise AP sites opposite all four bases. May also play a role in the regulation of mtDNA copy number by introducing a double-stranded break (DSB) at the mtDNA replication origin ori5, initiating the rolling-circle mtDNA replication.

Gene References into Functions

Both Ntg1 and its human homologue, NTHL1, can be SUMO-modified in response to oxidative stress. PMID: 27839712
the elements necessary for nuclear and mitochondrial localization of Ntg1 were identified, including a bipartite classical nuclear localization signal, a mitochondrial matrix targeting sequence and the classical nuclear protein import machinery. PMID: 20194111
loss of Ntg1p suppresses mitochondrial point mutation rates, frameshifts and recombination rates PMID: 16730479
The superb activity of the 8-oxoguanine glycosylases Fpg, hOGG1, and Ntg1 toward removal of FapyG and FapyA in vitro suggests that these enzymes may act upon these oxidized lesions in vivo. PMID: 18154319
The intracellular localization of the Saccharomyces cerevisiae N-glycosylase/AP lyases, Ntg1 and Ntg2, in response to nuclear and mitochondrial oxidative stress, was determined. PMID: 19029246

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Subcellular Location

Nucleus. Mitochondrion.

Protein Families

Nth/MutY family

Database Links

KEGG: sce:YAL015C

STRING: 4932.YAL015C

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NTG1 Antibody

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