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glnG Antibody

Datasheet
Code CSB-PA365286XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) glnG Polyclonal antibody
Uniprot No.
P0AFB8
Target Names
glnG
Alternative Names
glnG antibody; glnT antibody; ntrC antibody; b3868 antibody; JW3839 antibody; DNA-binding transcriptional regulator NtrC antibody; Nitrogen regulation protein NR(I) antibody; Nitrogen regulator I antibody; NRI antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) glnG protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
ELISA Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

glnG Proteins

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Target Background

Function
Member of the two-component regulatory system NtrB/NtrC, which controls expression of the nitrogen-regulated (ntr) genes in response to nitrogen limitation. Phosphorylated NtrC binds directly to DNA and stimulates the formation of open promoter-sigma54-RNA polymerase complexes. Activates transcription of many genes and operons whose products minimize the slowing of growth under nitrogen-limiting conditions, including genes coding for glutamine synthetase (glnA), transporters, amino acid permeases and catabolic enzymes.
Gene References into Functions
  1. Authors show that the dominant role of NtrC is to regulate glnA transcription and its own expression, indicating that the glutamine paradox is not due to NtrC-independent gene regulation. PMID: 24255125
  2. Results determine a possible pathway of interconversion between the active state and the inactive state of nitrogen regulatory protein C by targeted molecular dynamics simulations. PMID: 19576227
Subcellular Location
Cytoplasm.
Database Links
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