E(bx) Antibody

Code CSB-PA893829XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) E(bx) Polyclonal antibody
Uniprot No.
Target Names
E(bx)
Alternative Names
E(bx) antibody; CG32346 antibody; CG7022 antibody; Nurf301Nucleosome-remodeling factor subunit NURF301 antibody; Enhancer of bithorax antibody; Nucleosome-remodeling factor 215 kDa subunit antibody; NURF-215 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) E(bx) protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Histone-binding component of NURF (nucleosome remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. Required for homeotic gene expression, proper larval blood cell development, normal male X chromosome morphology, ecdysteroid signaling and metamorphosis.
Gene References into Functions
  1. The data show that, besides anticipated roles at enhancers, NURF interacts physically and functionally with the TRF2/DREF basal transcription factor to organize nucleosomes downstream of active promoters. PMID: 27046080
  2. required for stem cell maintenance in the Drosophila testis PMID: 20569693
  3. Chromatin immunoprecipitation localizes NURF to Ken-binding sites in hemocytes, suggesting that Ken recruits NURF. PMID: 18334252
  4. Study demonstrates that three NURF301 isoforms are expressed and that these encode functionally distinct NURF chromatin remodeling complexes. PMID: 19629165
Subcellular Location
Nucleus.
Protein Families
BPTF family
Database Links

KEGG: dme:Dmel_CG32346

STRING: 7227.FBpp0304412

UniGene: Dm.1449

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