NUCL1 Antibody

Code CSB-PA240419XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) NUCL1 Polyclonal antibody
Uniprot No.
Target Names
NUCL1
Alternative Names
NUCL1 antibody; PARL1 antibody; At1g48920 antibody; F27K7.6 antibody; Nucleolin 1 antibody; Protein NUCLEOLIN LIKE 1 antibody; AtNUC-L1 antibody; Protein PARALLEL 1 antibody; AtPARL1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana NUCL1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in pre-rRNA processing and ribosome assembly. Is associated with intranucleolar chromatin and pre-ribosomal particles and plays a role in controlling activation and repression of a specific subset of rRNA genes located in distinctive nucleolar organizer regions. Binds specifically rDNA chromatin and may be required to maintain rDNA chromatin structure, but is probably not required for the overall histone methylation status of 45S rRNA genes. Involved in leaf polarity establishment by functioning cooperatively with AS1 to repress abaxial genes ARF3, ARF4, KAN1, KAN2, YAB1 and YAB5, and the knox homeobox genes KNAT1, KNAT2, KNAT6, and STM to promote adaxial development in leaf primordia at shoot apical meristems at high temperatures.
Gene References into Functions
  1. NUC1 maintains cytosine methylations at specific sets of CpG sites in exon 6 of ARF3. PMID: 29415182
  2. Disruption of the AtNUC-L1 gene induces loss of symmetrical DNA methylation without affecting histone epigenetic marks at rRNA genes. PMID: 21124873
  3. PARL1 protein has roles similar to those of the yeast nucleolin in nuclear signal recognition, ribosomal processing, and ribosomal subunit accumulation. PMID: 17369435
Subcellular Location
Nucleus, nucleolus.
Tissue Specificity
Expressed in roots, leaves, shoots and flowers.
Database Links

KEGG: ath:AT1G48920

STRING: 3702.AT1G48920.1

UniGene: At.25056

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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