Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) nuoL Polyclonal antibody

Uniprot No.

P33607

Target Names

nuoL

Alternative Names

nuoL antibody; b2278 antibody; JW2273 antibody; NADH-quinone oxidoreductase subunit L antibody; EC 7.1.1.- antibody; NADH dehydrogenase I subunit L antibody; NDH-1 subunit L antibody; NUO12 antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) nuoL protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Gene References into Functions

NuoL is involved in the high efficiency pumping mechanism in complex I. PMID: 27118783
The results do not support the hypothesis that the lateral helix of the NuoL subunit functions like a piston. PMID: 26134569
To explore the functional relevance of the pseudosymmetric inverted-repeat structures of the antiporter-like subunits NuoL/M/N, we constructed a symmetry-related structure of a possible alternate-access state. PMID: 24778264
Data show that the mutation of the conserved amino acid D563(L), which is part of the horizontal helix of the Escherichia coli complex I subunit NuoL, leads to a reduced H(+)/e(-) stoichiometry. PMID: 22326235
NuoL was truncated at its C- and N-termini, which resulted in low production of a fragile Complex I with negligible activity. PMID: 22060017
the membrane subunit NuoL(ND5) is involved in the indirect proton pumping mechanism of Escherichia coli complex I PMID: 20826797
Data show that subunits NuoL and M jointly occupy a distal half of the membrane domain of respiratory complex I, separated by about 10nm from the interface with the peripheral arm. PMID: 17360196
An antiporter related subunit of NADH dehydrogenase I produced in Saccharomyces cerevisiae functions as transporter of Na+ and K+. PMID: 17583799

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Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

Complex I subunit 5 family

Database Links

KEGG: ecj:JW2273

STRING: 316385.ECDH10B_2440

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nuoL Antibody

Product Details

Customer Reviews and Q&A

Target Background