obst-E Antibody

Code CSB-PA131289XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) obst-E Polyclonal antibody
Uniprot No.
Target Names
obst-E
Alternative Names
obst-E antibody; CG11142 antibody; Protein obstructor-E antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) obst-E protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Chitin-binding protein that is important for the longitudinal contraction and lateral expansion of the larval cuticle during its conversion into the oval-shaped puparium case. Essential for survival to the second instar larval stage. Confers the orientated contractility and expandability of the larval cuticle by regulating the arrangement of chitin and the formation of supracellular ridges on the cuticle of third instar larvae. Essential for determining pupal body shape; required for the orientated shape change of the cuticle during metamorphosis which involves changes in the morphology of the supracellular ridges.; Mainly involved in regulating pupal shape.; Mainly involved in larvae survival, possibly by maintaining the normal morphology of the larval hindgut during development.
Gene References into Functions
  1. Obst-E regulates the arrangement of chitin and directs the formation of supracellular ridges on the larval cuticle. PMID: 28076349
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Tissue Specificity
[Isoform A]: Uniformly expressed throughout the cuticle of third instar larva.
Database Links

KEGG: dme:Dmel_CG11142

STRING: 7227.FBpp0078795

UniGene: Dm.2325

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