aceE Antibody

Code CSB-PA365296XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) aceE Polyclonal antibody
Uniprot No.
Target Names
aceE
Alternative Names
aceE antibody; b0114 antibody; JW0110 antibody; Pyruvate dehydrogenase E1 component antibody; PDH E1 component antibody; EC 1.2.4.1 antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) aceE protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).
Gene References into Functions
  1. thiamin cofactor communication in the Escherichia coli pyruvate dehydrogenase complex E1 component active centers is a "direct pathway" between the 4'-aminopyrimidine N1' atoms PMID: 20106967
  2. Observations suggest a mode of cooperativity in the E1 component that is consistent with the dynamical hypothesis shown to satisfactorily explain cooperativity in many well studied enzymes. PMID: 19801660
  3. Evidence is presented for the N-terminal region of pyruvate dehydrogenase complex E1 (PDHc-E1) subunit from E. coli playing a major role in the interaction with the PDHc-E2 subunit. PMID: 15518552
  4. Loop dynamics correlates with catalysis in the pyruvate dehydrogenase decarboxylase E1 component and represents the rate-limiting step for catalysis; overall dynamics of E1 creates a regulatory switch to streamline the catalytic steps in the complex. PMID: 18216265
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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