aceE Antibody

Datasheet

Code	CSB-PA365296XA01ENV
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Target Background

Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) aceE Polyclonal antibody

Uniprot No.

P0AFG8

Target Names

aceE

Alternative Names

aceE antibody; b0114 antibody; JW0110 antibody; Pyruvate dehydrogenase E1 component antibody; PDH E1 component antibody; EC 1.2.4.1 antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

A synthesized peptide derived from Escherichia coli (strain K12) aceE protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, Dot blot (guaranteed specific to the immunizing peptide)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

①. 100ug * KLH-conjugated peptide paired with 100ug * BSA-conjugated peptide (positive control);
②. 1ml*pre-immune serum (used as negative control) for free;
③. Rabbit polyclonal antibodies purified by Antigen Affinity;

Quality Guarantee

1. CUSABIO guarantees the antibody purity > 95% confirmed by SDS-PAGE.
2. CUSABIO guarantees the antibody titer > 1: 64,000 confirmed by ELISA.
3. CUSABIO guarantees to offer Peptide-specific Dot blot.
4. CUSABIO guarantees 100% risk-free: No qualified antibody, no charge.

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).

Gene References into Functions

thiamin cofactor communication in the Escherichia coli pyruvate dehydrogenase complex E1 component active centers is a "direct pathway" between the 4'-aminopyrimidine N1' atoms PMID: 20106967
Observations suggest a mode of cooperativity in the E1 component that is consistent with the dynamical hypothesis shown to satisfactorily explain cooperativity in many well studied enzymes. PMID: 19801660
Evidence is presented for the N-terminal region of pyruvate dehydrogenase complex E1 (PDHc-E1) subunit from E. coli playing a major role in the interaction with the PDHc-E2 subunit. PMID: 15518552
Loop dynamics correlates with catalysis in the pyruvate dehydrogenase decarboxylase E1 component and represents the rate-limiting step for catalysis; overall dynamics of E1 creates a regulatory switch to streamline the catalytic steps in the complex. PMID: 18216265

Database Links

KEGG: ecj:JW0110

STRING: 316385.ECDH10B_0094

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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aceE Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background