aceF Antibody

Datasheet

Code	CSB-PA006926XA01ENV
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) aceF Polyclonal antibody

Uniprot No.

P06959

Target Names

aceF

Alternative Names

aceF antibody; b0115 antibody; JW0111 antibody; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex antibody; EC 2.3.1.12 antibody; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex antibody; E2 antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) aceF protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Gene References into Functions

The inclusion of each additional domain of E2p strengthened the interaction with E1p, and the interaction was strongest with intact E2p. PMID: 24742683
Pyruvate dehydrogenase complex E2 (PDHc-E2)subunit protects N-terminal residues 1-55 of PDHc-E1 subunit selectively from trypsin digestion. PMID: 15518552
the dynamic loop at the active center of the Escherichia coli pyruvate dehydrogenase complex E1 component modulates substrate utilization and chemical communication with the E2 component PMID: 17635929
The surface loop of E1o is important to the structural integrity of the protein and together with Thr42 plays an important role in specifying the interaction of the lipoyl domain with its partner E1o in the E. coli 2OGDH complex. PMID: 17927566

Protein Families

2-oxoacid dehydrogenase family

Database Links

KEGG: ecj:JW0111

STRING: 316385.ECDH10B_0095

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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aceF Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background