ROMT-15 Antibody

Code CSB-PA896388XA01OFG
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Product Details

Full Product Name
Rabbit anti-Oryza sativa subsp. japonica (Rice) ROMT-15 Polyclonal antibody
Uniprot No.
Target Names
ROMT-15
Alternative Names
ROMT-15 antibody; COA20 antibody; Os08g0498100 antibody; LOC_Os08g38900 antibody; OsJ_27813 antibody; P0026F07.24 antibody; Tricin synthase 1 antibody; EC 2.1.1.175 antibody; Caffeoyl-CoA 3-O-methyltransferase ROMT15 antibody
Raised in
Rabbit
Species Reactivity
Oryza sativa subsp. japonica (Rice)
Immunogen
Recombinant Oryza sativa subsp. japonica (Rice) ROMT-15 protein
Immunogen Species
Oryza sativa subsp. japonica (Rice)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the stepwise methylation of tricetin to its 3'-mono- and 3',5'-dimethyl ethers. No 3',4',5'-trimethylated ester derivatives are produced. Can use caffeoyl-CoA, 5-hydroxyferulic acid, luteolin, tricetin, quercetin, myrcetin and 7,8-dihydroxyflavone as substrates, but not naringenin, apigenin or kaempferol. The 2,3-double bond and the O-dihydroxyl group of the substrate are both required for catalytic activity of the enzyme.
Gene References into Functions
  1. cloning of ROMT-15 and ROMT-17; ROMT-15 and -17 are cation-dependent and mutation of the predicted metal binding sites resulted in the loss of the enzyme activity PMID: 17943312
Subcellular Location
Nucleus.
Protein Families
Class I-like SAM-binding methyltransferase superfamily, Cation-dependent O-methyltransferase family, CCoAMT subfamily
Tissue Specificity
Ubiquitous. Highest expression in stems and roots.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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