ORC1A Antibody

Code CSB-PA325004XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ORC1A Polyclonal antibody
Uniprot No.
Target Names
ORC1A
Alternative Names
ORC1A antibody; At4g14700 antibody; dl3390w antibody; FCAALL.93 antibody; Origin of replication complex subunit 1A antibody; AtORC1a antibody; Origin recognition complex subunit 1a antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ORC1A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Essential protein. Component of the origin recognition complex (ORC) that binds origins of replication. It has a role in both chromosomal replication and mating type transcriptional silencing. Binds to the ARS consensus sequence (ACS) of origins of replication. H3K4me3 effector that regulates positively the transcription of a subset of genes.
Gene References into Functions
  1. ORC1a and ORC1b are regulated by the E2F/DP family of transcription factors and AtORC1a is preferentially expressed in endoreplicating cells. [ORC1a] PMID: 16179646
  2. ORC1 and Mcm3 proteins are required for DNA replication in protein extracts from human cells PMID: 16537544
  3. ORC1 functions as a transcriptional activator of a subset of genes, the promoters of which are preferentially bound by ORC1 PMID: 19171893
Subcellular Location
Nucleus.
Protein Families
ORC1 family
Tissue Specificity
Follow a cell-cycle regulation with a peak at the G1/S-phase. Mostly expressed in siliques, flowers and flower buds, and, to a lower extent, in roots, leaves and stems.
Database Links

KEGG: ath:AT4G14700

STRING: 3702.AT4G14700.1

UniGene: At.25357

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