ORTH1 Antibody

Code CSB-PA887784XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ORTH1 Polyclonal antibody
Uniprot No.
Target Names
ORTH1
Alternative Names
ORTH1 antibody; VIM3 antibody; At5g39550 antibody; MIJ24.3 antibody; E3 ubiquitin-protein ligase ORTHRUS 1 antibody; EC 2.3.2.27 antibody; Protein VARIANT IN METHYLATION 3 antibody; RING-type E3 ubiquitin transferase ORTHRUS 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ORTH1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
E3 ubiquitin-protein ligase. Participates in CpG methylation-dependent transcriptional regulation and epigenetic transcriptional silencing. Mediates ubiquitination with the E2 ubiquitin-conjugating enzymes UBC11, UBC8 and UBC8 homologs (e.g. UBC10, UBC11, UBC28 and UBC29) but not with UBC27, UBC30, UBC32, UBC34 and UBC36. Promotes methylation-mediated gene silencing leading, for example, to early flowering. Can bind to CpG, CpNpG, and CpNpN DNA motifs, with a strong preference for methylated forms, and with highest affinity for CpG substrate.
Gene References into Functions
  1. VIM proteins function in transcriptional regulation via their roles in the MET1 DNA methylation pathway. PMID: 24762702
  2. ORTH proteins are ubiquitin E3 ligases that regulate DNA methylation. The ORTH1 RING domain promotes ubiquitylation, and overexpression leads to an altered flowering time because of decreased methylation of FWA. PMID: 18643997
  3. VIM1, VIM2, and VIM3 have overlapping functions in maintenance of global CpG methylation and epigenetic transcriptional silencing. PMID: 18704160
Subcellular Location
Nucleus. Note=Broadly distributed in the nucleus and enriched in the heterochromatic chromocenters.
Tissue Specificity
Expressed in inflorescences and leaves.
Database Links

KEGG: ath:AT5G39550

STRING: 3702.AT5G39550.1

UniGene: At.30336

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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