osm-3 Antibody

Code CSB-PA343613XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans osm-3 Polyclonal antibody
Uniprot No.
Target Names
osm-3
Alternative Names
osm-3 antibody; M02B7.3 antibody; Osmotic avoidance abnormal protein 3 antibody; Kinesin-like protein osm-3 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans osm-3 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Kinesin motor protein which is required for the anterograde intraflagellar transport (IFT) along the middle segment of the sensory neuron cilia together with the kinesin II motor complex (composed of klp-11, klp-20 and kap-1) and on its own, is required for IFT along the distal segment. In addition, regulates the length of cilia. May have a role during neurogenesis and axonal transport.
Gene References into Functions
  1. OSM-3 mutations cause NPHP-4 dependent and independent phenotypes. PMID: 26863025
  2. Data show that kinesin-II and OSM-3 facilitate ciliary import and long-range transport. PMID: 26523365
  3. Dauer pheromone and gpa-3 signaling modulate the coordination of intraflagellar transport of kinesin-II and OSM-3. PMID: 20501698
  4. OSM-3 is an accessory intraflagellar transport (IFT) kinesin motor; subtle changes in its deployment or actions can contribute to differences in cilia morphology, cilia function, and sensory perception. PMID: 16492809
  5. Wild-type worms are induced to display an L1 (first larval stage) surface epitope at later larval stages when grown on an extract of spent culture medium; this was abolished in chemotaxis-defective osm-3 mutants. PMID: 16879619
  6. In this study, using a single-molecule fluorescence assay, the investigators show that bacterially expressed OSM-3 in solution does not move processively and displays low microtubule-stimulated adenosine triphosphatase (ATPase) activity. PMID: 17000874

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Subcellular Location
Cytoplasm, cytoskeleton. Cell projection, cilium. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm, cytoskeleton, cilium basal body.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, Kinesin II subfamily
Tissue Specificity
Expressed in an exclusive set of 26 chemosensory neurons whose dendritic endings are exposed to the external environment; six IL2 neurons of the inner labial sensilla, 8 pairs of amphid neurons in the head, and 2 pairs of phasmid neurons in the tail.
Database Links

KEGG: cel:CELE_M02B7.3

STRING: 6239.M02B7.3b

UniGene: Cel.12541

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