ospF Antibody

Code CSB-PA823791XA01SZB
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Product Details

Full Product Name
Rabbit anti-Shigella flexneri ospF Polyclonal antibody
Uniprot No.
Target Names
ospF
Alternative Names
ospF antibody; mkaD antibody; CP0010 antibody; pWR501_0013 antibody; SFLP011 antibody; Phosphothreonine lyase OspF antibody; EC 4.2.3.- antibody; Effector protein OspF antibody
Raised in
Rabbit
Species Reactivity
Shigella flexneri
Immunogen
Recombinant Shigella flexneri ospF protein
Immunogen Species
Shigella flexneri
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the removal of the phosphate group from the phosphothreonine in the mitogen-activated protein kinases such as MAPK2/ERK2, MAPK3/ERK1, MAPK8 and MAPK14 in an irreversible reaction, thus preventing the downstream phosphorylation of histone H3. This epigenetic modification results in inhibition of the transcription of a specific subset of proinflammatory genes, and ultimately to a reduced immune response against the invading pathogen. The diminished immune response enhances the bacterium's ability to disseminate and multiply within the host.
Gene References into Functions
  1. OspF has a role in neutralizing the MAPK pathway both by preventing its rephosphorylation and by inducing covalent modifications PMID: 28325837
  2. Shigella flexneri targets the HP1gamma subcode through the phosphothreonine lyase OspF. PMID: 25216677
Subcellular Location
Secreted. Note=Secreted via the type III secretion system (TTSS). Localizes in the nucleus of the infected cell.
Protein Families
Phosphothreonine lyase family
Database Links

KEGG: sfl:CP0010

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