Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ABI1 Polyclonal antibody

Uniprot No.

P49597

Target Names

ABI1

Alternative Names

ABI1 antibody; At4g26080 antibody; F20B18.190 antibody; Protein phosphatase 2C 56 antibody; AtPP2C56 antibody; EC 3.1.3.16 antibody; Protein ABSCISIC ACID-INSENSITIVE 1 antibody; Protein phosphatase 2C ABI1 antibody; PP2C ABI1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ABI1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Key component and repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane (Pos), drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth. During the stomatal closure regulation, modulates the inward calcium-channel permeability as well as the actin reorganization in guard cells in response to ABA. Involved in the resistance to the bacterial pathogen Pseudomonas syringae pv. tomato. Controls negatively fibrillin expression that is involved in mediating ABA-induced photoprotection. May be involved in ABA content regulation. Plays a role in the Pro accumulation in response to reduced water availability (low water potential). Required for the ABA negative regulation of the ethylene-induced hyponastic growth. Involved in acquired thermotolerance of root growth and seedling survival. Activates/represses SRK2E/OST1 in response to ABA-dependent stimuli, especially in stomata closure regulation involving SLAC1. Represses MAPKKK18 activity and promotes MAPKKK18 degradation by the proteasome pathway upon abscisic acid (ABA) treatment. Represses KIN10 activity by the specific dephosphorylation of its T-loop Thr-198, leading to a poststress inactivation of SnRK1 signaling. Restricts MAPKKK20 activity by dephosphorylation.

Gene References into Functions

Phosphatase ABI1 and okadaic acid-sensitive phosphatases of the PPP family are negative regulators of salt stress-activated SnRK2.4. PMID: 27297076
ABI1 acts to inhibit MAPKKK18 kinase activity, but also affects MAPKKK18 protein turnover via the ubiquitin-proteasome pathway. SnRK2.6 kinase also seems to be important for the regulation of MAPKKK18 function. PMID: 26852793
ABI1 regulates the activity and promotes proteasomal degradation of MKKK18. PMID: 26443375
The role of ABI1 in the activation of nuclear genes during transcription of chloroplast genes PMID: 25976841
There is direct cross-talk between C/N and non-canonical ABA signalling pathways, regulated by ABI1, in plants. PMID: 25795738
In the endodermis, misexpression of the ABA insensitive1-1 mutant protein, which dominantly inhibits abscisic acid (ABA) signaling, leads to a substantial recovery in lateral root (LR) growth under salt stress conditions. PMID: 23341337
Two dominant-negative mutations in ABA insensitive1 (abi1-1) and abi2-1 greatly reduced ROS production in mitochondria. PMID: 22652060
ROP11 protects ABI1 phosphatase activity from inhibition by RCAR1/PYL9 and thus negatively regulates abscissic acid signaling. PMID: 22251383
the molecular basis for PP2Cs-mediated inhibition of SnRK2s PMID: 22090030
study demonstrates ABI1 can interact with several PYR/PYL/RCAR family members, that PYR1-ABI1 interaction is rapidly stimulated by ABA & indicate new SnRK2 kinase-PYR/PYL/RCAR interactions in an emerging model for PYR/PYL/RCAR-mediated ABA signalling PMID: 19874541
HAB1 and the related PP2Cs ABI1 and ABI2 interact with OST1 in vivo, and mutations in the corresponding genes strongly affect OST1 activation by ABA. PMID: 19855047
The consequences of altered abscisic acid (ABA) sensitivity in gray poplar (Populus x canescens [Ait.] Sm.) development were examined by ectopic expression of the Arabidopsis (Arabidopsis thaliana) mutant abi1 (for abscisic acid insensitive1) gene. PMID: 19837818
the direct interaction between SRK2E/OST1 and ABI1 through Domain II plays a critical role in the control of stomatal closure PMID: 16365038
ABI1 and ABI2 control fibrillin expression that is involved in mediating ABAinduced photoprotection. [ABI1] PMID: 16571665
ABI1 works with HAB1 to negatively regulate ABA signaling. PMID: 16798945
AtBI1 plays a pivotal role as a highly conserved survival factor during ER stress that acts in parallel to the unfolded protein response pathway PMID: 18039663
abi1-1 acts as a hypermorphic allele, and ABI1 reprograms sensitivity towards ABA in the nucleus PMID: 18298671
regulatory component of abscissic acid (ABA)receptor 1(RCAR1, At1g01360) was shown to bind ABA, to mediate ABA-dependent inactivation of type 2C protein phosphatases (PP2Cs) ABI1 or ABI2 in vitro, and to antagonize PP2C action in planta PMID: 19407143
ABI1 plays the role of a general signal transducer linking abscisic acid and ethylene biosynthesis as well as signalling pathways to ozone stress tolerance. PMID: 19705149

Hide All

Subcellular Location

Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein. Note=Associated to the plasma membrane when in complex with PA, subsequently to ABA signaling.

Protein Families

PP2C family

Tissue Specificity

Expressed in seeds and seedlings. In roots, confined to lateral root caps and columella cells.

Database Links

KEGG: ath:AT4G26080

STRING: 3702.AT4G26080.1

UniGene: At.21332

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ABI1 Antibody

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