PPH1 Antibody

Code CSB-PA343428XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PPH1 Polyclonal antibody
Uniprot No.
Target Names
PPH1
Alternative Names
PPH1; PP2C57; TAP38; At4g27800; T27E11.40; Protein phosphatase 2C 57; AtPP2C57; Protein phosphatase 2C PPH1; PP2C PPH1; Thylakoid-associated phosphatase of 38 kDa
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana PPH1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Protein phosphatase specifically required for efficient dephosphorylation of the light-harvesting complex II outer antennae (LCHII) and transition from state 2 to state 1. State transition plays a central role in response to environmental changes and allows to adjust to changing light conditions via the redistribution of light excitation energy between photosystem II (PSII) and photosystem I (PSI) in a short time by relocating LHCII proteins (Probable). Mainly responsible for the dephosphorylation of Lhcb1 and Lhcb2 but not of the photosystem II core proteins.
Gene References into Functions
  1. The failure of the psal mutant to dock pLHCII to PSI induces excessive phosphorylation of LHCII, primarily due to a marked downregulation of the TAP38/PPH1 phosphatase occurring at post-transcriptional level. PMID: 26926011
  2. TAP38 is required for LHCII dephosphorylation and for the transition from state 2 to state 1 in A. thaliana. PMID: 20126264
  3. Data demonstrate that loss of PPH1 leads to an increase in the antenna size of photosystem I and to a strong impairment of state transitions. PMID: 20176943
  4. comparative genome-wide overview of the PP2C family in Arabidopsis and rice provides insights into the functions and regulatory mechanisms PMID: 19021904
Subcellular Location
[Isoform 1]: Membrane; Single-pass membrane protein.; [Isoform 2]: Plastid, chloroplast stroma.
Protein Families
PP2C family
Database Links

KEGG: ath:AT4G27800

STRING: 3702.AT4G27800.1

UniGene: At.23824

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