P4H13 Antibody

Code CSB-PA969613XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) P4H13 Polyclonal antibody
Uniprot No.
Target Names
P4H13
Alternative Names
P4H13 antibody; At2g23096 antibody; F21P24 antibody; Prolyl 4-hydroxylase 13 antibody; AtP4H13 antibody; EC 1.14.11.2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana P4H13 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in proline-rich peptide sequences of plant glycoproteins and other proteins. Hydroxyprolines are important constituent of many plant cell wall glycoproteins such as extensins, hydroxyproline-rich glycoproteins, lectins and arabinogalactan proteins. Possesses high affinity for leucine-rich repeat and proline-rich extensins of root cell walls that are essential for root hair development. Hydroxyprolines define the subsequent O-glycosylation sites by arabinosyltransferases which elongate the O-arabinosides on extensins.
Gene References into Functions
  1. P4H5, and to a lesser extent P4H2 and P4H13, are pivotal for root hair tip growth. P4H5 has in vitro preferred specificity for EXT substrates rather than for other HRGPs. And, by P4H promoter and protein swapping approaches, it is shown that P4H2 and P4H13 have interchangeable functions but cannot replace P4H5. [P4H13] PMID: 25655826
Subcellular Location
Endoplasmic reticulum membrane; Single-pass type II membrane protein.
Protein Families
P4HA family
Tissue Specificity
Expressed in epidermal root hair cells (trichoblasts) root hairless cells (atrichoblasts).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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