Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) P5CSA Polyclonal antibody

Uniprot No.

P54887

Target Names

P5CSA

Alternative Names

P5CSA antibody; P5CS1 antibody; At2g39800 antibody; T5I7.10 antibody; Delta-1-pyrroline-5-carboxylate synthase A antibody; P5CS A) [Includes: Glutamate 5-kinase antibody; GK antibody; EC 2.7.2.11 antibody; Gamma-glutamyl kinase); Gamma-glutamyl phosphate reductase antibody; GPR antibody; EC 1.2.1.41 antibody; Glutamate-5-semialdehyde dehydrogenase antibody; Glutamyl-gamma-semialdehyde dehydrogenase)] antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana P5CSA protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants.

Gene References into Functions

light regulates salt-induced transcriptional memory via the function of HY5 in maintaining H3K4me3 level at the memory gene; salt-induced transcriptional memory of P5CS1 is associated with the retention of increased H3K4me3 level at P5CS1 during the recovery stage PMID: 27930298
The results reveal a previously unknown link between Pro metabolism and phosphate nutrition and show that Pro biosynthesis is target of cross talk between Abscisic Acid (ABA) signaling and regulation of phosphate homeostasis through PHR1- and PHL1-mediated transcriptional activation of the P5CS1 gene. PMID: 28765275
Taken together, our findings reveal the novel function of FRI in enhancing drought resistance through its downstream P5CS1 pathway during water-deficit stress, which is dependent on its target, the FLC gene. PMID: 29175388
Evaluation of Salt Tolerance of Transgenic Tobacco Plants Bearing with P5CS1 Gene of Arabidopsis thaliana PMID: 27055296
P5CS1 and P5CS2 are expressed at similar levels and with the same pattern of expression in vegetative and floral shoot apical meristems as well as in axillary meristems. PMID: 19627555
Intron-mediated alternative splicing of Arabidopsis P5CS1 and its association with natural variation in proline and climate adaptation. PMID: 22615385
Results show that down-regulation of P5CS1 occurred in 35S-P5CS1 leaves, leading to proline reduction, and, in turn, impaired bolting and stunted growth. PMID: 18060533

Hide All

Protein Families

Glutamate 5-kinase family; Gamma-glutamyl phosphate reductase family

Database Links

KEGG: ath:AT2G39800

STRING: 3702.AT2G39800.1

UniGene: At.20482

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P5CSA Antibody

Product Details

Customer Reviews and Q&A

Target Background