pab2 Antibody

Code CSB-PA017365XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) pab2 Polyclonal antibody
Uniprot No.
Target Names
pab2
Alternative Names
pab2 antibody; SPBC16E9.12c antibody; Polyadenylate-binding protein 2 antibody; PABP-2 antibody; Poly(A)-binding protein 2 antibody; Poly(A)-binding protein II antibody; PABII antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) pab2 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Gene References into Functions
  1. Whereas PABPN1 strongly increases the activity of its cognate poly(A) polymerase in vitro, Pab2 was unable to stimulate Pla1 to any significant extent. PMID: 28096519
  2. Pab2 functions with Rrp6 to negatively control Tf2 mRNA accumulation. the absence of Pab2 attenuated the derepression of Tf2 transcription and the increased frequency of Tf2 mobilization caused by the deletion of abp1. PMID: 28007890
  3. Nab2 impedes Pab2/Rrp6-mediated decay by competing with Pab2 for polyadenylated transcripts in the nucleus. PMID: 24081329
  4. A proline-tyrosine nuclear localization signal is necessary and sufficient for nuclear import of Pab2. PMID: 23279110
  5. Pab2 and the nuclear exosome subunit, Rrp6 are the main factors involved in this polyadenylation-dependent pre-mRNA degradation pathway PMID: 21981922
  6. a model in which Mmi1 selectively targets meiotic transcripts for degradation via Pab2 and the exosome PMID: 20622014
  7. Pab2 promotes poly(A) tail trimming from pre-snoRNAs by recruiting the nuclear exosome. PMID: 20129053

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Subcellular Location
Nucleus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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