RIM101 Antibody

Code CSB-PA341415XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RIM101 Polyclonal antibody
Uniprot No.
Target Names
RIM101
Alternative Names
RIM101 antibody; RIM1 antibody; YHL027W antibody; pH-response transcription factor pacC/RIM101 antibody; Regulator of IME2 protein 1 antibody; pH-response regulator protein RIM101 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RIM101 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor that mediates regulation of both acid- and alkaline-expressed genes in response to ambient pH. At alkaline ambient pH, activates transcription of alkaline-expressed genes (including RIM101 itself), mainly by repressing transcriptional repressors of those genes, and represses transcription of acid-expressed genes. Required for meiosis, sporulation and invasive growth.
Gene References into Functions
  1. These results suggest that the Rim101 pathway is important for the adaptation to ER stress by compensating for alterations in plasma membrane lipid asymmetry induced by ER stress. PMID: 27803246
  2. We conclude that summation operator1278b may harbor a variant(s) that buffers against widespread transcriptional dysregulation upon introduction of a non-native RIM101 allele, emphasizing the importance of accounting for genetic background . PMID: 26751950
  3. A role of Rim101 in modulation of vacuolar acidity necessary for selenite detoxification is discovered. PMID: 25239548
  4. Results found that activity of the Rsp5 ubiquitin ligase was required for recruiting the downstream molecules to the plasma membrane, suggesting that ubiquitination mediates Rim101 signaling at the plasma membrane. PMID: 25002535
  5. Pho85 regulates the nuclear accumulation of active Rim101, possibly via phosphorylation PMID: 20382759
  6. Rim101 pathway mediates colony patterning by responding to cell-to-cell pH signals. PMID: 20038633
  7. RIM101 pathway contributes to cell wall assembly and its function becomes essential in the absence of SIt2. PMID: 16524906
  8. RIM101 expression is shown to be required to counteract propionic acid-induced cytosolic acidification and for proper vacuolar acidification and cell wall structure. PMID: 19220866
  9. The double mutant slt2Delta rim101Delta displays altered chitin rings, with a significant reduction in the levels of chitin. PMID: 19633265

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
PacC/RIM101 family
Database Links

KEGG: sce:YHL027W

STRING: 4932.YHL027W

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