PAD1 Antibody

Code CSB-PA333822XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PAD1 Polyclonal antibody
Uniprot No.
Target Names
PAD1
Alternative Names
PAD1 antibody; POF1 antibody; YDR538W antibody; Flavin prenyltransferase PAD1 antibody; mitochondrial antibody; EC 2.5.1.129 antibody; Phenylacrylic acid decarboxylase 1 antibody; PAD antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PAD1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for the ferulic acid decarboxylase FDC1/ubiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN. Involved in the decarboxylation of phenylacrylic acids like ferulic acid, p-coumaric acid or cinnamic acid, producing the corresponding vinyl derivatives which play the role of aroma metabolites. Also involved in the degradation of the food preservative sorbic acid (2,4-hexadienoic acid) to a volatile hydrocarbon, 1,3-pentadiene. Not essential for ubiquinone synthesis. Can rescue Q biosynthesis in E.coli strains lacking UbiX. Has mRNA binding activity.
Gene References into Functions
  1. Ferulic acid decarboxylase catalyzes the decarboxylation of phenylacrylic acid using a newly identified cofactor, prenylated flavin mononucleotide (prFMN). The proposed mechanism involves the formation of a putative pentacyclic intermediate formed by a 1,3 dipolar cyclo-addition of prFMN with the alpha-beta double bond of the substrate, which serves to activate the substrate toward decarboxylation. PMID: 28753302
  2. The kinetics and substrate requirements of prenyl-flavin synthase were determined. Dimethylallyl pyrophosphate is the prenyl donor. The kinetics of the reaction are extremely slow: kcat = 12.2 +/- 0.2 h(-1), and KM for dimethylallyl pyrophosphate = 9.8 +/- 0.7 muM. The reaction of FMN was described well by first-order kinetics with a kapp of 17.4 +/- 1.1 h(-1). scPFS. PMID: 29232110
  3. PAD1 synthesizes a cofactor required by FDC (ferulic acid decarboxylase) for decarboxylase activity. PMID: 25647642
  4. POF1 is an ATPase that is likely involved in the protein degradation pathway. PMID: 22204397
  5. examination of the contribution of the PAD1 gene to the decarboxylation of phenylacrylic acid using gene disruptants and the corresponding transformants in which the disrupted genes were recovered. PMID: 20471595
  6. Degrades the food preservative sorbic acid (2,4-hexadienoic acid) to a volatile hydrocarbon, identified by gas chromatography mass spectrometry as 1,3-pentadine. PMID: 17766451

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Subcellular Location
Mitochondrion.
Protein Families
UbiX/PAD1 family
Database Links

KEGG: sce:YDR538W

STRING: 4932.YDR538W

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