mbt Antibody

Code CSB-PA894288XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) mbt Polyclonal antibody
Uniprot No.
Target Names
mbt
Alternative Names
mbt antibody; CG18582 antibody; Serine/threonine-protein kinase PAK mbt antibody; EC 2.7.11.1 antibody; Protein mushroom bodies tiny antibody; p21-activated kinase-related protein antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) mbt protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in neurogenesis of the adult central nervous system, and together with Cdc42, regulates photoreceptor cell morphogenesis. Phosphorylates exogenous substrates when activated by Cdc42.
Gene References into Functions
  1. Pak4 regulates adherens junction accumulation. Pak4 promotes Par3 retention at the zonula adherens. PMID: 27052178
  2. Mbt regulates neuroblast number throughout postembryonic brain development. PMID: 23571212
  3. role for Mbt as a downstream effector of Cdc42 in photoreceptor cell morphogenesis PMID: 12490550
  4. Constitutive activation of Mbt not only disturbs the actin cytoskeleton but also affects adherens junction organisation indicating a requirement of the protein in cell adhesion dependent processes during photoreceptor cell differentiation. PMID: 17097274
  5. Activation of the human Mbt homologue PAK4 leads to anchorage-independent growth and provide a functional link between a PAK protein and the cadherin-catenin complex. PMID: 18636970

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Subcellular Location
Cell junction, adherens junction. Cell membrane; Peripheral membrane protein. Note=Apical membrane sites of adherens junctions of developing photoreceptor cells.
Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily
Tissue Specificity
Expressed in adult brain and eye. High levels detected in developing photoreceptor cells and future bristle cells, and lower levels in cone and pigment cells, as detected in third instar eye imaginal disks (at protein level).
Database Links

KEGG: dme:Dmel_CG18582

STRING: 7227.FBpp0074060

UniGene: Dm.4406

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