MPAO1 Antibody

Code CSB-PA524626XA01ZAX
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Product Details

Full Product Name
Rabbit anti-Zea mays (Maize) MPAO1 Polyclonal antibody
Uniprot No.
Target Names
MPAO1
Alternative Names
MPAO1 antibody; MPAO antibody; PAO antibody; Polyamine oxidase 1 antibody; EC 1.5.3.14 antibody; EC 1.5.3.15 antibody
Raised in
Rabbit
Species Reactivity
Zea mays (Maize)
Immunogen
Recombinant Zea mays (Maize) MPAO1 protein
Immunogen Species
Zea mays (Maize)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Flavoenzyme involved in polyamine back-conversion (Ref.4, PubMed:16331971). Catalyzes the oxidation of the secondary amino group of polyamines, such as spermine, spermidine and their acetyl derivatives (Ref.4, PubMed:16331971). Plays an important role in the regulation of polyamine intracellular concentration (Probable).
Gene References into Functions
  1. Underexpression of Zea mays polyamine oxidase (ZmPAO) correlates with increased thermotolerance of the photosynthetic machinery and improved biomass accumulation, accompanied by enhanced levels of the enzymatic and non-enzymatic antioxidants, whereas ZmPAO overexpressors exhibit significant impairment of thermotolerance. PMID: 28886452
  2. deregulation of the apoplastic PAO can be further examined as a potential approach to breed plants with enhanced/reduced tolerance to abiotic stress with minimal associated trade-offs. PMID: 28135604
  3. data suggest that H2O2 produced through PA catabolism by ZmPAO1 plays an important role in tumor development during the maize-U. maydis interaction. PMID: 26926794
  4. Rsults indicate that the HOH309-K300 couple plays a major role in multiple steps of ZmPAO catalytic mechanism, such as correct substrate binding geometry as well as FAD reduction and reoxidation kinetics. PMID: 21205212
  5. Data indicate the importance of lysine300 and probable importance of water309 to the mechanism of flavin reduction in maize polyamine oxidase. PMID: 16331971
  6. This study evaluated the effect of wounding on polyamine oxidase expression in the outer tissues of the maize mesocotyl and provide evidence that its activity, protein, and mRNA levels increased in response to wounding as well as jasmonic acid treatment. PMID: 17993545
  7. MPAO contributes to abscisic acid-induced cytosolic antioxidant defense through hydrogen peroxide, a Spermidine catabolic product. PMID: 19261065
  8. PAO contributes to sustain maize leaf elongation under saline stress. PMID: 19717530

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Subcellular Location
Secreted, extracellular space, apoplast. Secreted, cell wall.
Protein Families
Flavin monoamine oxidase family
Database Links

KEGG: zma:541983

STRING: 4577.GRMZM2G034152_P01

UniGene: Zm.300

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