PAP1 Antibody

Code CSB-PA335470XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PAP1 Polyclonal antibody
Uniprot No.
Target Names
PAP1
Alternative Names
PAP1 antibody; YKR002WPoly(A) polymerase antibody; PAP antibody; EC 2.7.7.19 antibody; Polynucleotide adenylyltransferase antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PAP1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Polymerase component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB.
Gene References into Functions
  1. results suggest a novel mechanism for controlling Pap1 activity, and possible models invoking these newly-discovered interactions are discussed. PMID: 22575652
  2. TFIIB interaction with the CF1 complex and Pap1 is crucial for gene looping and transcriptional regulation. PMID: 21835917
  3. new poly(A) polymerase complex that contains Air1p or Air2p as potential RNA-binding subunits PMID: 15828860
Subcellular Location
Nucleus.
Protein Families
Poly(A) polymerase family
Database Links

KEGG: sce:YKR002W

STRING: 4932.YKR002W

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